Composite

Part:BBa_K2549023:Design

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)


ZF21.16-VP64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has zinc finger DNA-binding protein ZF21.16 (Part:BBa_K2549046), G4S linker (Part:BBa_K2549053), nuclear localization signal SV40NLS (Part:BBa_K2549054), and transcription activator VP64 (Part:BBa_K2549057).


Source

From IDT (gBlock), human codon optimized